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| 產(chǎn)地 | 進(jìn)口、國產(chǎn) |
| 品牌 | 上海莼試 |
| 保存條件 | Store at -20 °C |
| 貨號(hào) | CS12098 |
| 應(yīng)用范圍 | WB=1:100-500 ELISA=1:500-1000 IP=1:20-100 IHC-P=1:100-500 IHC-F=1:100-500 IF=1:100-500 |
| CAS編號(hào) | |
| 抗體名 | Anti-SLC37A4/G6PT2 |
| 克隆性 | 是 |
| 靶點(diǎn) | 詳見說明書 |
| 適應(yīng)物種 | 詳見說明書 |
| 形態(tài) | 詳見說明書 |
| 宿主 | 詳見說明書 |
| 亞型 | IgG |
| 標(biāo)識(shí)物 | 詳見說明書 |
| 濃度 | 1mg/1ml% |
| 免疫原 | KLH conjugated synthetic peptide derived from human G6PT2 |
產(chǎn)品訂購信息：
英文名稱 Anti-SLC37A4/G6PT2
中文名稱 葡萄糖-6磷酸轉(zhuǎn)運(yùn)蛋白抗體規(guī)格
別 名 G6PT2; GSD1b; GSD1c; GSD1d; TRG19; G6PT1; G6PT3; Glucose-5-phosphate transporter; Glucose-6-phosphatase, transport (glucose) protein 3 antibody; Glucose-6-phosphatase, transport (glucose-6-phosphate) protein 1; Glucose-6-phosphatase, transport (phosphate/pyrophosphate) protein 2; Glucose-6-phosphate translocase; Glucose-6-phosphate transporter 1; Microsomal glucose-6-phosphate transporter; Solute carrier family 37 (glucose-6-phosphate transporter), member 4; MGC15729; PRO0685; G6PT1_HUMAN.
濃 度 1mg/1ml
規(guī) 格 0.2ml/200μg
抗體來源 Rabbit
克隆類型 polyclonal
交叉反應(yīng) Human, Mouse, Rat, Dog, Pig, Cow
產(chǎn)品類型 一抗
研究領(lǐng)域 細(xì)胞生物 免疫學(xué) 轉(zhuǎn)錄調(diào)節(jié)因子 轉(zhuǎn)運(yùn)蛋白
蛋白分子量 predicted molecular weight: 46kDa
性 狀 Lyophilized or Liquid
免 疫 原 KLH conjugated synthetic peptide derived from human G6PT2
亞 型 IgG
純化方法 affinity purified by Protein A
儲(chǔ) 存 液 0.01M PBS, pH 7.4 with 10 mg/ml BSA and 0.1% Sodium azide
葡萄糖-6磷酸轉(zhuǎn)運(yùn)蛋白抗體規(guī)格 產(chǎn)品應(yīng)用 WB=1:100-500 ELISA=1:500-1000 IP=1:20-100 IHC-P=1:100-500 IHC-F=1:100-500 IF=1:100-500
（石蠟切片需做抗原修復(fù)）
not yet tested in other applications.
optimal dilutions/concentrations should be determined by the end user.
保存條件 Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20°C. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4 °C.
Important Note This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.
產(chǎn)品介紹 SLC37A4 transports glucose-6-phosphate from the cytoplasm to the lumen of the endoplasmic reticulum. It forms a complex with glucose-6-phosphatase which is responsible for glucose production through glycogenolysis and gluconeogenesis. Hence, it plays a central role in homeostatic regulation of blood glucose levels.
Function : Transports glucose-6-phosphate from the cytoplasm to the lumen of the endoplasmic reticulum. Forms with glucose-6-phosphatase the complex responsible for glucose production through glycogenolysis and gluconeogenesis. Hence, it plays a central role in homeostatic regulation of blood glucose levels.
Subcellular Location : Endoplasmic reticulum membrane; Multi-pass membrane protein
Tissue Specificity : Mostly expressed in liver and kidney
DISEASE : Defects in SLC37A4 are the cause of glycogen storage disease type 1B (GSD1B) [MIM:232220]. GSD1B is a metabolic disorder characterized by impairment of terminal steps of glycogenolysis and gluconeogenesis. GSD1 patients manifest a wide range of clinical symptoms and biochemical abnormalities, including hypoglycemia, severe hepatomegaly due to excessive accumulation of glycogen, kidney enlargement, growth retardation, lactic acidemia, hyperlipidemia, and hyperuricemia. GSD1B patients also present a tendency towards infections associated with neutropenia, relapsing aphthous gingivostomatitis, and inflammatory bowel disease.
Defects in SLC37A4 are the cause of glycogen storage disease type 1C (GSD1C) [MIM:232240].
Defects in SLC37A4 are the cause of glycogen storage disease type 1D (GSD1D) [MIM:232240].
Similarity : Belongs to the major facilitator superfamily. Organophosphate:Pi antiporter (OPA) (TC 2.A.1.4) family.
Anti-Phospho-HSL (Ser552)/FITC 熒光素標(biāo)記磷酸化荷爾蒙敏感脂肪酶抗體IgGMulti-class antibodies規(guī)格： 0.2ml
Anti-PI3K/P85 alpha/FITC 熒光素標(biāo)記磷脂酰肌醇激酶抗體IgGMulti-class antibodies規(guī)格： 0.2ml
Rhesus antibody Rh ASP/AKAP associated Sperm Protein 相關(guān)蛋白AKAP抗體 規(guī)格 0.2ml
Donkey Anti-Guinea pig IgG/PE-CY5 PE-CY5標(biāo)記的驢抗豚鼠IgG 0.1ml
Glucose Oxidase 英文名稱： 葡萄糖氧化酶抗體 0.2ml
Rhesus antibody Rh Rabbit Anti-mouse IgG/PE-Cy3 PE-Cy3標(biāo)記的兔抗小鼠IgG 規(guī)格 0.1ml
Anti-PI3K/P85 alpha/FITC 熒光素標(biāo)記磷脂酰肌醇激酶抗體IgGMulti-class antibodies規(guī)格： 0.2ml
Anti-DC-SIGN/CD209/CLEC4L/FITC 熒光素標(biāo)記CD209抗體IgGMulti-class antibodies規(guī)格： 0.2ml
Anti-Phospho-PKM2 (Tyr105) /FITC 熒光素標(biāo)記磷酸化酸激酶M2抗體IgGMulti-class antibodies規(guī)格： 0.2ml
Rhesus antibody Rh CaMK2 beta/CaMK2 gamma 鈣/鈣調(diào)素依賴蛋白激酶2b/2γ抗體 規(guī)格 0.1ml
Cyclin D3(C-term) 周期素D3抗原 0.5mg
Hoxb3 英文名稱： 同源盒蛋白HoxB3抗體 0.2ml
Rhesus antibody Rh SEZ6L 樣相關(guān)蛋白6抗體 規(guī)格 0.2ml
Anti-Phospho-PKM2 (Tyr105) /FITC 熒光素標(biāo)記磷酸化酸激酶M2抗體IgGMulti-class antibodies規(guī)格： 0.2ml
NA(Human neuraminidase) ELISA Kit 人神經(jīng)氨酸酶Multi-class antibodies規(guī)格： 48T
Anti-NEDD4L/NEDD4-2 泛素連接酶NEDD4-2抗體Multi-class antibodies規(guī)格： 0.2ml
Rhesus antibody Rh IL-6 白介素6 規(guī)格 0.1ml
MMP-3 ELISA kit 大鼠基質(zhì)金屬蛋白酶3 96T
phospho-PRKCZ(Thr500) 英文名稱： 磷酸化蛋白激酶C(T500)抗體 0.2ml
C8orf55 英文名稱： 8號(hào)染色體開放閱讀框55抗體 0.2ml
Anti-NEDD4L/NEDD4-2 泛素連接酶NEDD4-2抗體Multi-class antibodies規(guī)格： 0.2ml
HAVCR2 Others Mouse 小鼠 TIM3 / HAVCR2 人細(xì)胞裂解液 (陽性對(duì)照)
小鼠紋狀體神經(jīng)元MN-s
人胚;MRC-5
DMS153細(xì)胞，人小細(xì)胞細(xì)胞 大鼠腎上腺嗜鉻細(xì)胞瘤細(xì)胞,PC12細(xì)胞 CM-H127人晶狀體上皮細(xì)胞完全培養(yǎng)基100mL
PANC-1(人*癌細(xì)胞) 5×106cells/瓶×2
Promocell C-28020 Hematopoietic ProgenitorMedium, 造血祖細(xì)胞培養(yǎng)基(即用型) 100ml
CL-0432RTE(大鼠氣管上皮細(xì)胞)5×106cells/瓶×2
IL23 Others Mouse 小鼠 IL-23 / IL23A & IL12B Heterodimer 人細(xì)胞裂解液 (陽性對(duì)照)
人腸微內(nèi)皮細(xì)胞RNAHIMEC miRNA5 μg
RBE細(xì)胞，人細(xì)胞 小鼠艾氏腹水瘤細(xì)胞,ECA細(xì)胞 人羊膜上皮細(xì)胞
M14(人細(xì)胞) 5×106cells/瓶×2
NHEK.f pooled Pellet 正常人表皮角質(zhì)細(xì)胞團(tuán)塊(少年者，混合來源) > 1 mio.cells 內(nèi)皮細(xì)胞培養(yǎng)基ECM
葡萄糖-6磷酸轉(zhuǎn)運(yùn)蛋白抗體規(guī)格 CL-0430RS1(大鼠皮膚成纖維樣細(xì)胞)5×106cells/瓶×2
IL22RA1 Others Canine 狗 IL22RA1 / IL22R 人細(xì)胞裂解液 (陽性對(duì)照)
人食道上皮細(xì)胞RNAHEEpiC miRNA5 μg
兔間變表皮鱗株;VX2 細(xì)胞，22RV1細(xì)胞 MDCK superdome細(xì)胞，狗腎細(xì)胞隆突型
人永生化淋巴細(xì)胞;CNLMG-B5537LC
HA Others H3N2 甲型 H3N2 (A/Aichi/2/1968) 血凝素HA1 (Hemagglutinin) 人細(xì)胞裂解液 (陽性對(duì)照)
抗體的生物素化標(biāo)記實(shí)驗(yàn)要點(diǎn)：
1. 葡萄糖-6磷酸轉(zhuǎn)運(yùn)蛋白抗體規(guī)格 如在反應(yīng)混合液中有疊氮鈉或游離氨基存在,會(huì)抑制標(biāo)記反應(yīng)。因此,蛋白質(zhì)在反應(yīng)前要對(duì) 0.1mol/L碳酸氫鈉緩沖液或0.5mol/L硼酸緩沖液充分透析；
2.所用的NHSB及待生物素化蛋白質(zhì)之間的分子比按蛋白質(zhì)表面的ε-氨基的密度會(huì)有所不同,選擇不當(dāng)則影響標(biāo)記的效率,應(yīng)先用幾個(gè)不同的分子比來篩選最適條件；
3.用NHSB量過量也是不利的,抗原的結(jié)合位點(diǎn)可能因此被封閉,導(dǎo)致抗體失活；
4.由于抗體的氨基不易接近可能造成生物素化不足,此時(shí)可加入去污劑如 Triton x-100, Tween20等；
5.當(dāng)游離ε-氨基(賴氨酸殘基的氨基)存在于抗體的抗原結(jié)合位點(diǎn)時(shí),或位于酶的催化位點(diǎn)時(shí),生物素化會(huì)降低或損傷抗體蛋白的結(jié)合力或活性；
6.生物素還可能與不同的功能基團(tuán),如羰基、氨基、巰基、異咪唑基及苯酚基,也可與糖基共價(jià)結(jié)合；
7.交聯(lián)反應(yīng)后,應(yīng)充分透析,否則,殘余的生物素會(huì)對(duì)生物素化抗體與親和素的結(jié)合產(chǎn)生競爭作用；
8.在細(xì)胞的熒光標(biāo)記實(shí)驗(yàn)中,中和親和素的本底低,但由于鏈霉親和素含有少量正電荷,故對(duì)某些細(xì)胞可導(dǎo)致高本底。
抗體的鑒定：
1）葡萄糖-6磷酸轉(zhuǎn)運(yùn)蛋白抗體規(guī)格 抗體的效價(jià)鑒定：不管是用于診斷還是用于,制備抗體的目的都是要求較高效價(jià)。不同的抗原制備的抗體,要求的效價(jià)不一。鑒定效價(jià)的方法很多,包括有試管凝集反應(yīng),瓊脂擴(kuò)散試驗(yàn),酶聯(lián)免疫吸附試驗(yàn)等。常用的抗原所制備的抗體一般都有約成的鑒定效價(jià)的方法,以資比較。如制備抗抗體的效價(jià),一般就采用瓊脂擴(kuò)散試驗(yàn)來鑒定。
2）抗體的特異性鑒定：抗體的特異性是指與相應(yīng)抗原或近似抗原物質(zhì)的識(shí)別能力。抗體的特異性高,它的識(shí)別能力就強(qiáng)。衡量特異性通常以交叉反應(yīng)率來表示。交叉反應(yīng)率可用競爭抑制試驗(yàn)測定。以不同濃度抗原和近似抗原分別做競爭抑制曲線,計(jì)算各自的結(jié)合率,求出各自在IC50時(shí)的濃度,并按公式計(jì)算交叉反應(yīng)率。
如果所用抗原濃度IC50濃度為pg/管,而一些近似抗原物質(zhì)的IC50濃度幾乎是無窮大時(shí),表示這一抗血清與其他抗原物質(zhì)的交叉反應(yīng)率近似為0,即該血清的特異性較好。
3）抗體親和力：是指抗體和抗原結(jié)合的牢固程度。親和力的高低是由抗原分子的大小,抗體分子的結(jié)合位點(diǎn)與抗原決定簇之間立體構(gòu)型的合適度決定的。有助于維持抗原抗體復(fù)合物穩(wěn)定的分子間力有氫鍵,疏水鍵,側(cè)鏈相反電荷基因的庫侖力,范德華力和空間斥力。親和力常以親和常數(shù)K表示,K的單位是L/mol。抗體親和力的測定對(duì)抗體的篩選,確定抗體的用途,驗(yàn)證抗體的均一性等均有重要意義。
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