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| 產(chǎn)地 | 進口、國產(chǎn) |
| 品牌 | 上海莼試 |
| 保存條件 | Store at -20 °C |
| 貨號 | CS12044 |
| 應(yīng)用范圍 | WB=1:100-500 ELISA=1:500-1000 IP=1:20-100 IHC-P=1:100-500 IHC-F=1:100-500 IF=1:100-500 |
| CAS編號 | |
| 抗體名 | Anti-UAP56/BAT1 |
| 克隆性 | |
| 靶點 | 詳見說明書 |
| 適應(yīng)物種 | 詳見說明書 |
| 形態(tài) | 詳見說明書 |
| 宿主 | 詳見說明書 |
| 亞型 | IgG |
| 標識物 | 詳見說明書 |
| 濃度 | 1mg/1ml% |
| 免疫原 | KLH conjugated synthetic peptide derived from human BAT1 |
產(chǎn)品訂購信息：
英文名稱 Anti-UAP56/BAT1
中文名稱 ATP-依賴的RNA解旋酶p47抗體說明書
別 名 56 kDa U2AF65-associated protein; ATP-dependent RNA helicase p47; B(0,+)-type amino acid transporter 1; BAT1; Bat1a; DEAD box protein UAP56; Glycoprotein-associated amino acid transporter b0,+AT1; HLA-B-associated transcript 1 protein; HLA-B-associated transcript 1A; HLA-B-associated transcript-1; p47; Solute carrier family 7 member 9; Spliceosome RNA helicase BAT1; UAP56; 56 kDa U2AF65-associated protein; ATP-dependent RNA helicase p47.
濃 度 1mg/1ml
規(guī) 格 0.1ml/100μg 0.2ml/200μg
抗體來源 Rabbit
克隆類型 polyclonal
交叉反應(yīng) Human, Mouse, Rat, Dog, Pig, Cow
產(chǎn)品類型 一抗
研究領(lǐng)域 免疫學 信號轉(zhuǎn)導 轉(zhuǎn)錄調(diào)節(jié)因子
蛋白分子量 predicted molecular weight: 49kDa
性 狀 Lyophilized or Liquid
免 疫 原 KLH conjugated synthetic peptide derived from human BAT1
亞 型 IgG
純化方法 affinity purified by Protein A
儲 存 液 0.01M PBS, pH 7.4 with 10 mg/ml BSA and 0.1% Sodium azide
ATP-依賴的RNA解旋酶p47抗體說明書 產(chǎn)品應(yīng)用 WB=1:100-500 ELISA=1:500-1000 IP=1:20-100 IHC-P=1:100-500 IHC-F=1:100-500 IF=1:100-500
（石蠟切片需做抗原修復）
not yet tested in other applications.
optimal dilutions/concentrations should be determined by the end user.
保存條件 Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20°C. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4 °C.
Important Note This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.
產(chǎn)品介紹 UAP56 belongs to the DEAD box family of RNA-dependent ATPases that mediate ATP hydrolysis during pre-mRNA splicing. UAP56 is an essential splicing factor required for association of U2 small nuclear ribonucleoprotein with pre-mRNA, and also plays an important role in mRNA export from the nucleus to the cytoplasm.
Function : [FUNCTION] Component of the THO subcomplex of the TREX complex. The TREX complex specifically associates with spliced mRNA and not with unspliced pre-mRNA. It is recruited to spliced mRNAs by a transcription-independent mechanism. Binds to mRNA upstream of the exon-junction complex (EJC) and is recruited in a splicing- and cap-dependent manner to a region near the 5' end of the mRNA where it functions in mRNA export. The recruitment occurs via an interaction between ALYREF/THOC4 and the cap-binding protein NCBP1. DDX39B functions as a bridge between ALYREF/THOC4 and the THO complex. The TREX complex is essential for the export of Kaposi's sarcoma-associated herpesvirus (KSHV) intronless mRNAs and infectious virus production. The recruitment of the TREX complex to the intronless viral mRNA occurs via an interaction between KSHV ORF57 protein and ALYREF/THOC4. Splice factor that is required for the first ATP-dependent step in spliceosome assembly and for the interaction of U2 snRNP with the branchpoint. Has both RNA-stimulated ATP binding/hydrolysis activity and ATP-dependent RNA unwinding activity. Even with the stimulation of RNA, the ATPase activity is weak. Can only hydrolyze ATP but not other NTPs. The RNA stimulation of ATPase activity does not have a strong preference for the sequence and length of the RNA. However, ssRNA stimulates the ATPase activity much more strongly than dsRNA. Can unwind 5' or 3' overhangs or blunt end RNA duplexes in vitro. The ATPase and helicase activities are not influenced by U2AF2 and ALYREF/THOC4.
Subunit : Homodimer, and heterodimer with DDX39A. Component of the THO complex, which is composed of THOC1, THOC2, THOC5, THOC6 and THOC7. Together with THOC3, ALYREF/THOC4 and DDX39B, THO forms the transcription/export (TREX) complex. Component of the spliceosome. Interacts directly with U2AF2. Interacts directly with ALYREF/THOC4 and is necessARy for ALYREF/THOC4 recruitment to spliced mRNA. Interacts with RBM8A, RNPS1 and SRRM1. Interacts with FYTTD1/UIF and THOC1. Interacts with human cytomegalovirus/HHV-5 protein UL69. Interacts with MX1.
Subcellular Location : Nucleus. Nucleus speckle. Cytoplasm. Note=Can translocate to the cytoplasm in the presence of MX1.
Similarity : Belongs to the DEAD box helicase family. DECD subfamily.
Contains 1 helicase ATP-binding domain.
Contains 1 helicase C-terminal domain.
Database links : UniProtKB/Swiss-Prot: Q13838.1
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Rhesus antibody Rh ASIC1/BNaC2 酸敏感離子通道1抗體 規(guī)格 0.1ml
Donkey Anti-Guinea pig IgG/PE-Cy5.5 PE-Cy5.5標記的驢抗豚鼠IgG 0.1ml
Phospho-Glucocorticoid Receptor (Ser226) 英文名稱： 磷酸化糖皮質(zhì)激素受體抗體 0.1ml
Rhesus antibody Rh Rabbit Anti-mouse IgG/FITC FITC標記的兔抗小鼠IgG 規(guī)格 0.3ml
Anti-PIM1/FITC 熒光素標記前病毒整合位點蛋白1抗體IgGMulti-class antibodies規(guī)格： 0.2ml
Anti-GAPDH/FITC 熒光素標記3-磷酸甘油醛脫氫酶IgGMulti-class antibodies規(guī)格： 0.2ml
Anti-NR1/NMDAR1/GLUR1/FITC 熒光素標記谷氨酸受體1抗體IgGMulti-class antibodies規(guī)格： 0.2ml
Rhesus antibody Rh BPGM 紅細胞2,3 - 二磷酸甘油酸合成酶抗體 規(guī)格 0.2ml
ICAD(Inhibitor of Caspase-Activated DNase ) Caspase 激活的脫氧核糖核酸酶抑制劑(抗原) 0.5mg
HOXA3 英文名稱： 同源盒基因HOXA3蛋白抗體 0.1ml
Rhesus antibody Rh Resistin 抵抗素抗體 規(guī)格 0.1ml
Anti-NR1/NMDAR1/GLUR1/FITC 熒光素標記谷氨酸受體1抗體IgGMulti-class antibodies規(guī)格： 0.2ml
CD62E(Human E-Selectin) ELISA kit 人E選擇素Multi-class antibodies規(guī)格： 48T
Anti-GSK-3 Beta (CT) GSK-3β抗體葡萄糖合成激酶-3β抗體Multi-class antibodies規(guī)格： 0.2ml
Rhesus antibody Rh MMP24/ MMP25/MMP21 基質(zhì)金屬蛋白酶-24抗體 規(guī)格 0.1ml
PG-A(Human Pepsinogen A) ELISA Kit 人胃蛋白酶原A 96T
phospho-Proteasome 20S beta 7(Ser214) 英文名稱： 磷酸化蛋白酶體PSMβ7抗體 0.2ml
c8orf84 英文名稱： 8號染色體開放閱讀框84抗體 0.2ml
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TGFBR2 Others Cynomolgus 食蟹猴 TGFBR2 人細胞裂解液 (陽性對照)
人成纖維細胞;HFF
F344大鼠骨髓間質(zhì)干細胞 F344 rat bone marrow mesenchymal stem cells
NW38細胞，人細胞 鼠李糖乳桿菌 人神經(jīng)膠質(zhì)細胞瘤細胞;U251
ANGPT2 Protein Mouse 重組小鼠 Angiopoietin-2 / ANG2 / ANGPT2 蛋白 (His 標簽)
JEG-3(人細胞) 5×106cells/瓶×2 食蟹猴 IL-21R / Ierleukin-21 Receptor 人細胞裂解液 (陽性對照)
CL-0446SUP-B15(人Ph+急淋細胞系)5×106cells/瓶×2
CD40 Others Canine 狗 CD40 / TNFRSF5 人細胞裂解液 (陽性對照)
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抗體的生物素化標記實驗要點：
1. ATP-依賴的RNA解旋酶p47抗體說明書 如在反應(yīng)混合液中有疊氮鈉或游離氨基存在,會抑制標記反應(yīng)。因此,蛋白質(zhì)在反應(yīng)前要對 0.1mol/L碳酸氫鈉緩沖液或0.5mol/L硼酸緩沖液充分透析；
2.所用的NHSB及待生物素化蛋白質(zhì)之間的分子比按蛋白質(zhì)表面的ε-氨基的密度會有所不同,選擇不當則影響標記的效率,應(yīng)先用幾個不同的分子比來篩選最適條件；
3.用NHSB量過量也是不利的,抗原的結(jié)合位點可能因此被封閉,導致抗體失活；
4.由于抗體的氨基不易接近可能造成生物素化不足,此時可加入去污劑如 Triton x-100, Tween20等；
5.當游離ε-氨基(賴氨酸殘基的氨基)存在于抗體的抗原結(jié)合位點時,或位于酶的催化位點時,生物素化會降低或損傷抗體蛋白的結(jié)合力或活性；
6.生物素還可能與不同的功能基團,如羰基、氨基、巰基、異咪唑基及苯酚基,也可與糖基共價結(jié)合；
7.交聯(lián)反應(yīng)后,應(yīng)充分透析,否則,殘余的生物素會對生物素化抗體與親和素的結(jié)合產(chǎn)生競爭作用；
8.在細胞的熒光標記實驗中,中和親和素的本底低,但由于鏈霉親和素含有少量正電荷,故對某些細胞可導致高本底。
抗體的鑒定：
1）ATP-依賴的RNA解旋酶p47抗體說明書 抗體的效價鑒定：不管是用于診斷還是用于,制備抗體的目的都是要求較高效價。不同的抗原制備的抗體,要求的效價不一。鑒定效價的方法很多,包括有試管凝集反應(yīng),瓊脂擴散試驗,酶聯(lián)免疫吸附試驗等。常用的抗原所制備的抗體一般都有約成的鑒定效價的方法,以資比較。如制備抗抗體的效價,一般就采用瓊脂擴散試驗來鑒定。
2）抗體的特異性鑒定：抗體的特異性是指與相應(yīng)抗原或近似抗原物質(zhì)的識別能力?？贵w的特異性高,它的識別能力就強。衡量特異性通常以交叉反應(yīng)率來表示。交叉反應(yīng)率可用競爭抑制試驗測定。以不同濃度抗原和近似抗原分別做競爭抑制曲線,計算各自的結(jié)合率,求出各自在IC50時的濃度,并按公式計算交叉反應(yīng)率。
如果所用抗原濃度IC50濃度為pg/管,而一些近似抗原物質(zhì)的IC50濃度幾乎是無窮大時,表示這一抗血清與其他抗原物質(zhì)的交叉反應(yīng)率近似為0,即該血清的特異性較好。
3）抗體親和力：是指抗體和抗原結(jié)合的牢固程度。親和力的高低是由抗原分子的大小,抗體分子的結(jié)合位點與抗原決定簇之間立體構(gòu)型的合適度決定的。有助于維持抗原抗體復合物穩(wěn)定的分子間力有氫鍵,疏水鍵,側(cè)鏈相反電荷基因的庫侖力,范德華力和空間斥力。親和力常以親和常數(shù)K表示,K的單位是L/mol?？贵w親和力的測定對抗體的篩選,確定抗體的用途,驗證抗體的均一性等均有重要意義。
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