|
| 產(chǎn)地 | 進口、國產(chǎn) |
| 品牌 | 上海莼試 |
| 保存條件 | Store at -20 °C |
| 貨號 | CS11983 |
| 應(yīng)用范圍 | ELISA=1:500-1000 IHC-P=1:100-500 IHC-F=1:100-500 Flow-Cyt=1:100-500 ICC=1:100-500 IF=1:100-500 |
| CAS編號 | |
| 抗體名 | Anti-UNC5H3/UNC5C |
| 克隆性 | |
| 靶點 | 詳見說明書 |
| 適應(yīng)物種 | 詳見說明書 |
| 形態(tài) | 詳見說明書 |
| 宿主 | 詳見說明書 |
| 亞型 | IgG |
| 標(biāo)識物 | 詳見說明書 |
| 濃度 | 1mg/1ml% |
| 免疫原 | KLH conjugated synthetic peptide derived from human UNC5H3 |
產(chǎn)品訂購信息：
英文名稱 Anti-UNC5H3/UNC5C
中文名稱 神經(jīng)突起生長誘導(dǎo)因子受體UNC5B抗體品牌
別 名 Alternative nameshomolog of C. elegans transmembrane receptor Unc5; Netrin receptor UNC5C; Protein unc 5 homolog C; Unc 5 homolog 3; Unc 5 homolog C; Unc5 (C.elegans homolog) c; UNC5H3; UNC5C_HUMAN.
濃 度 1mg/1ml
規(guī) 格 0.2ml/200μg
抗體來源 Rabbit
克隆類型 polyclonal
交叉反應(yīng) Human, Mouse, Rat, Chicken, Dog, Pig, Cow, Horse, Rabbit, Sheep, 1
產(chǎn)品類型 一抗
研究領(lǐng)域 細胞生物 發(fā)育生物學(xué) 神經(jīng)生物學(xué)
蛋白分子量 predicted molecular weight: 99kDa
性 狀 Lyophilized or Liquid
免 疫 原 KLH conjugated synthetic peptide derived from human UNC5H3
亞 型 IgG
純化方法 affinity purified by Protein A
儲 存 液 Preservative: 15mM Sodium Azide, Constituents: 1% BSA, 0.01M PBS, pH 7.4
神經(jīng)突起生長誘導(dǎo)因子受體UNC5B抗體品牌 產(chǎn)品應(yīng)用 ELISA=1:500-1000 IHC-P=1:100-500 IHC-F=1:100-500 Flow-Cyt=1:100-500 ICC=1:100-500 IF=1:100-500
（石蠟切片需做抗原修復(fù)）
not yet tested in other applications.
optimal dilutions/concentrations should be determined by the end user.
保存條件 Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20°C. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4 °C.
Important Note This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.
產(chǎn)品介紹 The UNC5H family of proteins act as transmembrane receptors for netrin-1 and play a crucial role in axon guidance and migration of neural cells. Additionally, UNC5H receptors induce apoptosis when cleaved by a caspase, producing an intracellular fragment containing a death domain. This activity is blocked by the binding of netrin-1. In the absence of netrin-1, UNC5H receptors act as tumor suppressors by inhibiting anchorage-independent growth and invasion, but mutation of these receptors provides a potential mechanism for tumorigenicity. The expression of UNC5H receptors is down-regulated in multiple cancers, including colorectal, breast, ovary, uterus, stomach, lung, and kidney cancers. UNC5H3, also known as UNC5C, plays an important role in the development of spinal accessory motor neurons. It is also involved in mediating the repulsive action for netrin-1 and it serves as a stop signal for migratory cells.
Function : UNC5C is a receptor for netrin required for axon guidance. It mediates axon repulsion of neuronal growth cones in the developing nervous system upon ligand binding. Axon repulsion in growth cones may be caused by its association with DCC that may trigger signaling for repulsion. It is also involved in corticospinal tract axon guidances independently of DCC. It also acts as a dependence receptor required for apoptosis induction when not associated with netrin ligand. There are two named isoforms.
Subunit : Interacts with the cytoplasmic part of DCC
Subcellular Location : Membrane; Single-pass type I membrane protein
Tissue Specificity : Mainly expressed in brain. Also expressed in kidney. Not expressed in developing or adult lung.
Post-translational modifications : Phosphorylated on different cytoplasmic tyrosine residues. Phosphorylation of Tyr-568 leads to an interaction with PTPN11 phosphatase, suggesting that its activity is regulated by phosphorylation/dephosphorylation. Tyrosine phosphorylation is netrin-dependent (By similarity).
Proteolytically cleaved by caspases during apoptosis. The cleavage does not take place when the receptor is associated with netrin ligand. Its cleavage by caspases is required to induce apoptosis
Similarity : Belongs to the unc-5 family.
Contains 1 death domain.
Contains 1 Ig-like (immunoglobulin-like) domain.
Contains 1 Ig-like C2-type (immunoglobulin-like) domain. [SIMILARITY] Contains 2 TSP type-1 domains. [SIMILARITY] Contains 1 ZU5 domain.
Database links : UniProtKB/Swiss-Prot: O95185.2
Parvalbumin 微白蛋白/細小清蛋白抗原Multi-class antibodies規(guī)格： 0.5mg
Anti-BADH2 BADH2抗體(水稻)Multi-class antibodies規(guī)格： 0.2ml
Rhesus antibody Rh Phospho-5-Lipoxygenase(Ser663) 磷酸化5-脂氧合酶抗體 規(guī)格 0.1ml
Keratin19 濃縮液 0.1ml 進口分裝
UBE2A 英文名稱： 泛素結(jié)合酶E2蛋白A抗體 0.2ml
CHST10 英文名稱： 碳水化合物磺基轉(zhuǎn)移酶10抗體 0.1ml
Anti-BADH2 BADH2抗體(水稻)Multi-class antibodies規(guī)格： 0.2ml
ASK1/MAPKKK5 細胞凋亡信號調(diào)節(jié)激酶1/原活化蛋白激酶激酶激酶5抗原Multi-class antibodies規(guī)格： 0.5mg
Anti-AMPK alpha 1 腺苷單磷酸活化蛋白激酶α1抗體Multi-class antibodies規(guī)格： 0.1ml
Rhesus antibody Rh Phospho-BRCA1(Ser1189) 磷酸化癌易感基因1抗體 規(guī)格 0.1ml
Tris-EDTA緩沖液(50X) 50ml pH 9.0,高背景著色
VASP 英文名稱： 擴張刺激磷蛋白抗體 0.1ml
Defensin alpha 6 英文名稱： 防御素α6抗體 0.2ml
Anti-AMPK alpha 1 腺苷單磷酸活化蛋白激酶α1抗體Multi-class antibodies規(guī)格： 0.1ml
Anti-Phospho-SHIP2 (Ser576) /FITC 熒光素標(biāo)記磷酸化蛋白酪氨酸磷酸酶2抗體IgGMulti-class antibodies規(guī)格： 0.2ml
AATK peptide 細胞凋亡關(guān)聯(lián)酪氨酸激酶抗原Multi-class antibodies規(guī)格： 0.5mg
神經(jīng)激肽B受體抗體 Anti-NKR 0.1ml
Somatostatin 28 英文名稱： 生長抑素抗體 0.1ml
Endothelin 2 英文名稱： 內(nèi)皮素2抗體 0.1ml
Rhesus antibody Rh Phospho-p56Dok2(Tyr351) 磷酸化D酪氨酸激酶衰減蛋白2抗體 規(guī)格 0.1ml
AATK peptide 細胞凋亡關(guān)聯(lián)酪氨酸激酶抗原Multi-class antibodies規(guī)格： 0.5mg
大鼠可溶性內(nèi)皮細胞蛋白C受體(sEPCR)ELISA試劑盒 ，英文名： sEPCR ELISA Kit
人內(nèi)皮細胞生長因子受體1(VEGFR-1)ELISA檢測試劑盒HumanVascuoarendothelialcellgrowthfactorreceptor1,VEGFR-1ELISAkit 96T/48T
三聚胺ELISA快速檢測試劑盒 Melamine ELISA Kit
CLIAKitforOPG(HumanOsteoprotegerin)ELISAKIT人骨保護素規(guī)格：48T/96T
糖化血清白蛋白(GSP)化學(xué)比色法定量檢測試劑盒50次
ELISAKitAO人橙規(guī)格：48T/96T
大鼠白細胞介素1α(IL-1α)ELISA試劑盒 ，英文名： IL-1α ELISA Kit
Lens culinaris agglinin (LCA) ELISA Kit 扁豆凝集素(LCA)ELISA試劑盒
Mousegrowthhormonereleasingpeptide-Ghrelin,GHRP-GhrelinELISAKit 小鼠生長激素釋放肽ghrelin(GHRP-Ghrelin)ELISA試劑盒 96T/48T 進口分裝
CLIAKitforHumanNon-PhosphorylatedInsulin-likegrowthfactorbindingprotein1ELISAKit人未磷酸化類生長因子結(jié)合蛋白-1規(guī)格：48T/96T
同位素真菌/酵母細胞蛋白核酸結(jié)合凝膠遷移電泳分析試劑盒20次
ELISAKitsICAM-1大鼠可溶性細胞間粘附分子1規(guī)格：48T/96T
神經(jīng)突起生長誘導(dǎo)因子受體UNC5B抗體品牌 大鼠催乳素誘導(dǎo)蛋白(PIP)ELISA試劑盒 ，英文名： PIP ELISA Kit
Rat ierleukin 9 (IL-9) ELISA Kit 大鼠白介素9(IL-9)ELISA試劑盒
RatProstaglandinE2,PG-E2ELISAKit 大鼠素E2(PGE2)ELISA試劑盒 96T/48T 進口分裝
CLIAKitforCTLA-4/CD152(HumancytotoxicTlymphocyteassociatedaigen4)ELISAKit人細胞毒性T淋巴細胞相關(guān)抗原4規(guī)格：48T/96T
細胞糖原化學(xué)水解比色法定量檢測試劑盒20次
Rateosinophilcationicprotein,ECPELISAKit大鼠嗜酸性粒細胞陽離子蛋白(ECP)ELISA試劑盒規(guī)格：96T/48T
抗體的生物素化標(biāo)記實驗要點：
1. 神經(jīng)突起生長誘導(dǎo)因子受體UNC5B抗體品牌 如在反應(yīng)混合液中有疊氮鈉或游離氨基存在,會抑制標(biāo)記反應(yīng)。因此,蛋白質(zhì)在反應(yīng)前要對 0.1mol/L碳酸氫鈉緩沖液或0.5mol/L硼酸緩沖液充分透析；
2.所用的NHSB及待生物素化蛋白質(zhì)之間的分子比按蛋白質(zhì)表面的ε-氨基的密度會有所不同,選擇不當(dāng)則影響標(biāo)記的效率,應(yīng)先用幾個不同的分子比來篩選最適條件；
3.用NHSB量過量也是不利的,抗原的結(jié)合位點可能因此被封閉,導(dǎo)致抗體失活；
4.由于抗體的氨基不易接近可能造成生物素化不足,此時可加入去污劑如 Triton x-100, Tween20等；
5.當(dāng)游離ε-氨基(賴氨酸殘基的氨基)存在于抗體的抗原結(jié)合位點時,或位于酶的催化位點時,生物素化會降低或損傷抗體蛋白的結(jié)合力或活性；
6.生物素還可能與不同的功能基團,如羰基、氨基、巰基、異咪唑基及苯酚基,也可與糖基共價結(jié)合；
7.交聯(lián)反應(yīng)后,應(yīng)充分透析,否則,殘余的生物素會對生物素化抗體與親和素的結(jié)合產(chǎn)生競爭作用；
8.在細胞的熒光標(biāo)記實驗中,中和親和素的本底低,但由于鏈霉親和素含有少量正電荷,故對某些細胞可導(dǎo)致高本底。
抗體的鑒定：
1）神經(jīng)突起生長誘導(dǎo)因子受體UNC5B抗體品牌 抗體的效價鑒定：不管是用于診斷還是用于,制備抗體的目的都是要求較高效價。不同的抗原制備的抗體,要求的效價不一。鑒定效價的方法很多,包括有試管凝集反應(yīng),瓊脂擴散試驗,酶聯(lián)免疫吸附試驗等。常用的抗原所制備的抗體一般都有約成的鑒定效價的方法,以資比較。如制備抗抗體的效價,一般就采用瓊脂擴散試驗來鑒定。
2）抗體的特異性鑒定：抗體的特異性是指與相應(yīng)抗原或近似抗原物質(zhì)的識別能力?？贵w的特異性高,它的識別能力就強。衡量特異性通常以交叉反應(yīng)率來表示。交叉反應(yīng)率可用競爭抑制試驗測定。以不同濃度抗原和近似抗原分別做競爭抑制曲線,計算各自的結(jié)合率,求出各自在IC50時的濃度,并按公式計算交叉反應(yīng)率。
如果所用抗原濃度IC50濃度為pg/管,而一些近似抗原物質(zhì)的IC50濃度幾乎是無窮大時,表示這一抗血清與其他抗原物質(zhì)的交叉反應(yīng)率近似為0,即該血清的特異性較好。
3）抗體親和力：是指抗體和抗原結(jié)合的牢固程度。親和力的高低是由抗原分子的大小,抗體分子的結(jié)合位點與抗原決定簇之間立體構(gòu)型的合適度決定的。有助于維持抗原抗體復(fù)合物穩(wěn)定的分子間力有氫鍵,疏水鍵,側(cè)鏈相反電荷基因的庫侖力,范德華力和空間斥力。親和力常以親和常數(shù)K表示,K的單位是L/mol。抗體親和力的測定對抗體的篩選,確定抗體的用途,驗證抗體的均一性等均有重要意義。
|
|
|
|
|
